Biophysics and Molecular Materials

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Dr. Pippig Diana

Dr. Diana Pippig


Lehrstuhl für Angewandte Physik
und Center for NanoScience
Amalienstr. 54
80799 München

Room: 408
Phone: +49 (0) 89/ 2180-3132
Fax: +49 (0) 89/ 2180-2050

Further Information

Research Interests

We are interested in studying single molecules by means of force and fluorescence spectroscopy. To aid examining these intricate systems we continuously try to improve strategies to control and handle single molecules by utilizing functional groups, specific coupling chemistry and site-selective labeling. For example, we established AFM-based Single-Molecule Cut & Paste (SMC&P) as a highly sophisticated method to place individual biomolecules into defined assembly areas.
We have a strong interest in studying force sensory or even force-activated proteins like muscle kinases. For their characterization we implement hybrid approaches utilizing atomic force spectroscopy in combination with TIRF microscopy.

Curriculum Vitae

since 01/2011

Ludwig-Maximilians-University, München
Postdoctoral researcher and group leader in the group of Prof. Dr. H. E. Gaub

  • single molecule studies, force sensory kinases
  • protein biochemistry, surface and coupling chemistry, force and fluorescence spectroscopy, nanobiotechnology, molecular biology

10/2010 – 12/2010

Ludwig-Maximilians-University, München – Gene Center
Postdoctoral researcher in the group of Prof. Dr. K.-P. Hopfner

  • pattern recognition receptors of the innate immune system
  • molecular biology, protein and RNA biochemistry, structural biology / x-ray crystallography, immunology

2007 – 2011

Ludwig-Maximilians-University, München – Gene Center
PhD graduate student in biochemistry in the group of Prof. Dr. K.-P. Hopfner

  • receptors of the innate immune system
  • molecular biology, protein and RNA biochemistry, structural biology / x-ray crystallography, immunology

International Max Planck Research School for Molecular and Cellular Life Sciences (IMPRS-LS) – Member of the PhD program


“Structural and Biochemical Characterization of Pattern Recognition Receptors of the Human Innate Immune System that Respond to Pathogenic Nucleic Acids.”


University of Otago, New Zealand
Research assistant in the group of Dr. S. Wilbanks

  • chaperones, pathogenic cytochrome C phenotypes
  • molecular biology, protein biochemistry, structural biology / x-ray crystallography, fluorescence spectroscopy

2001 – 2005

University of Bayreuth
Diploma student in biochemistry

Diploma Thesis:

“Biochemical and Crystallographic Characterization of the SPOA0172-Gene Product of Silicibacter pomeroyi”, conducted in the group of Prof. H. Dobbek at the University of Bayreuth


  • Baumann, F., Bauer, S. M., Milles, L.F., Alexandrovich, A., Gaub, H.E., Pippig, D.A. (2015) Monovalent Strep-Tactin for strong and site-specific tethering in nano spectroscopy, Nature Nanotechnology, doi: 10.1038/nnano.2015.231
  • Aschenbrenner, D., Baumann, F., Milles, L.F., Pippig, D.A., Gaub, H.E. (2015) C-5 Propynyl Modifications Enhance the Mechanical Stability of DNA, Chemphyschem, doi: 10.1002/cphc.201500193
  • Baumann, F., Heucke, S.F., Pippig, D.A., Gaub, H.E. (2015) Tip localization of an atomic force microscope in transmission microscopy with nanoscale precision, Rev Sci Instrum, 86(3)
  • Aschenbrenner, D., Pippig, D.A., Klamecka, K., Limmer, K., Leonhardt, H., Gaub, H.E. (2014) Parallel force assay for protein-protein interactions, PLoS One, 9(12)
  • Otten, M., Ott, W., Jobst, M.A., Milles, L.F., Verdorfer, T., Pippig, D.A., Nash, M.A., Gaub, H.E. (2014) From genes to protein mechanics on a chip, Nat Methods, 11(11):1127-30
  • Pippig, D.A., Baumann, F., Strackharn, M., Aschenbrenner, D., and Gaub, H.E. (2014) Protein-DNA Chimeras for Nano Assembly, ACS Nano, 8(7):6551-5
  • Limmer, K., Pippig, D.A., Aschenbrenner, D., and Gaub, H.E. (2014) A force-based, parallel assay for the quantification of protein-DNA interactions, PLoS ONE, 9 (2)
  • Strackharn, M.*, Pippig, D.A.*, Meyer, P., Stahl, S.W., and Gaub, H.E. (2012) Nanoscale Arrangement of Proteins by Single-Molecule Cut-and-Paste, JACS, Vol. 134 (37), 15193-15196.
  • Pippig, D. A., Hellmuth, J. C., Cui, S., Kirchhofer, A., Lammens, K., Lammens, A., Schmidt, A., Rothenfusser, S., and Hopfner, K. P. (2009) The regulatory domain of the RIG-I family ATPase LGP2 senses double-stranded RNA, Nucleic Acids Res 37, 2014-2025.
  • Morison, I. M., Cramer Borde, E. M., Cheesman, E. J., Cheong, P. L., Holyoake, A. J., Fichelson, S., Weeks, R. J., Lo, A., Davies, S. M., Wilbanks, S. M., Fagerlund, R. D., Ludgate, M. W., da Silva Tatley, F. M., Coker, M. S., Bockett, N. A., Hughes, G., Pippig, D. A., Smith, M. P., Capron, C., and Ledgerwood, E. C. (2008) A mutation of human cytochrome c enhances the intrinsic apoptotic pathway but causes only thrombocytopenia, Nat Genet 40, 387-389.
  • Jeoung, J. H.*, Pippig, D. A.*, Martins, B. M., Wagener, N., and Dobbek, H. (2007) HTHP: a novel class of hexameric, tyrosine-coordinated heme proteins, J Mol Biol 368, 1122-1131.

* shared first authorship